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Article

Bispecific antibody process development: Assembly and purification of knob and hole bispecific antibodies.

Biotechnology progress - 1 Mar 2018

Giese Glen, Williams Ambrose, Rodriguez Maricel, Persson Josefine

Abstract excerpt

Production of knob and hole dual light chain bispecific antibodies poses several unique challenges for development of a feasible industrial scale manufacturing process. We developed an efficient process for the assembly and purification of knob and hole dual light chain bispecific antibodies. Two distinct half-antibodies targeting two different antigens were expressed separately in Escherichia coli cells and...

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PMID: 29193902
DOI: 10.1002/btpr.2590

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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