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Article

Discovery of Zika Virus Dependency and Restriction Factors Using Flow-Based Arrayed CRISPR Screening for Identification of Targets (FACS-IT).

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2020

McDougall William M, Kandpal Manish, Perreira Jill M, Brass Abraham L

Abstract excerpt

The discovery and implementation of CRISPR/Cas9 tools in pooled genetic screens have allowed for the rapid, high-fidelity identification of host-virus interactions. However, pooled CRISPR screening has significant limitations in its ability both to perform cell biology and plate reader-based screens and to find alleles that result in intermediate-strength phenotypes. Here we introduce an arrayed CRISPR screening...

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PMID: 32367370
DOI: 10.1007/978-1-0716-0581-3_17

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

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