Article
High-throughput screening assay for D-amino acid oxidase.
Analytical biochemistry - 15 Mar 2008
Khoronenkova Svetlana V, Tishkov Vladimir I
Abstract excerpt
A membrane-based high-throughput screening (HTS) assay for active D-amino acid oxidase (DAAO) in liquid samples as well as in intact Escherichia coli cells has been developed and optimized. The detection limit of the assay was less than 1 ng per sample. The method proposed can be used for quantitative DAAO determination in the range of 0.13 to 3.60 ng enzyme per probe. The protocol was successfully tested to...
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