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Article

Real-time observation of CRISPR spacer acquisition by Cas1-Cas2 integrase.

Nature structural & molecular biology - 1 May 2020

Budhathoki Jagat B, Xiao Yibei, Schuler Gabriel, Hu Chunyi, Cheng Alexander, Ding Fran, Ke Ailong

Abstract excerpt

Cas1 integrase associates with Cas2 to insert short DNA fragments into a CRISPR array, establishing nucleic acid memory in prokaryotes. Here we applied single-molecule FRET methods to the Enterococcus faecalis (Efa) Cas1-Cas2 system to establish a kinetic framework describing target-searching, integration, and post-synapsis events. EfaCas1-Cas2 on its own is not able to find the CRISPR repeat in the CRISPR array;...

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Identifiers

PMID: 32367067
DOI: 10.1038/s41594-020-0415-7
PMCID: PMC7241818

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