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CRISPR/Cas System and Factors Affecting Its Precision and Efficiency

24 Nov 2021

Abstract excerpt

The diverse applications of genetically modified cells and organisms require more precise and efficient genome-editing tool such as clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas). The CRISPR/Cas system was originally discovered in bacteria as a part of adaptive-immune system with multiple types. Its engineered versions involve multiple host DNA-repair pathways in...

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PMID: 34901007
DOI: 10.3389/fcell.2021.761709

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