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Article

Phenomics-Based Quantification of CRISPR-Induced Mosaicism in Zebrafish.

Cell systems - 25 Mar 2020

Watson Claire J, Monstad-Rios Adrian T, Bhimani Rehaan M, Gistelinck Charlotte, Willaert Andy, Coucke Paul, Hsu Yi-Hsiang, Kwon Ronald Y

Abstract excerpt

Genetic mosaicism can manifest as spatially variable phenotypes that vary from site to site within an organism. Here, we use imaging-based phenomics to quantitate phenotypes at many sites within the axial skeleton of CRISPR-edited G0 zebrafish. Through characterization of loss-of-function cell clusters in the developing skeleton, we identify a distinctive size distribution shown to arise from clonal fragmentation...

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Source

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Identifiers

PMID: 32191876
DOI: 10.1016/j.cels.2020.02.007
PMCID: PMC7213258

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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