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Article

Nanobody affinity improvement: Directed evolution of the anti-ochratoxin A single domain antibody.

International journal of biological macromolecules - 15 May 2020

Wang Xuerou, Chen Qi, Sun Zhichang, Wang Yidan, Su Benchao, Zhang Chenghui, Cao Hongmei, Liu Xing

Abstract excerpt

The characteristics of single domain and ease of gene manipulation of the single domain antibody (sdAb) make it suitable for affinity maturation in vitro. Since the affinity of antibodies can influence the immunoassays' sensitivity, a nanobody (Nb), the anti-ochratoxin A sdAb (AOA-sdAb), was herein selected as the model antibody to explore feasible approach for improving its affinity. Homology modeling and...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 32084462
DOI: 10.1016/j.ijbiomac.2020.02.180

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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