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Article

Anti-idiotypic single-chain variable fragment antibody partially mimic the functionally spatial structure of Cry2Aa toxin.

Analytical biochemistry - 15 Jul 2021

Lin Manman, Liu Yuan, Zhang Xiao, Zhong Jianfeng, Hu Xiaodan, Xu Chongxin, Xie Yajing, Zhang Cunzheng, Liang Ying, Liu Xianjin, Lin Johnson

Abstract excerpt

The anti-idiotypic antibody is widely used in the field of immunology to simulate structural features or even induce the biological activity of antigens. In this study, we obtained seven anti-idiotypic single-chain variable fragments (scFv) antibodies of Cry2Aa toxin from a phage-displayed mutant library constructed using error-prone PCR technique. A mutant designated 2-12B showed the best binding ability amongst...

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Identifiers

PMID: 33932355
DOI: 10.1016/j.ab.2021.114222

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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