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Double-digest RAD-sequencing: do pre- and post-sequencing protocol parameters impact biological results?

Molecular genetics and genomics : MGG - 1 Mar 2021

Cumer Tristan, Pouchon Charles, Boyer Frédéric, Yannic Glenn, Rioux Delphine, Bonin Aurélie, Capblancq Thibaut

Abstract excerpt

Next-generation sequencing technologies have opened a new era of research in population genetics. Following these new sequencing opportunities, the use of restriction enzyme-based genotyping techniques, such as restriction site-associated DNA sequencing (RAD-seq) or double-digest RAD-sequencing (ddRAD-seq), has dramatically increased in the last decade. From DNA sampling to SNP calling, the laboratory and...

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PMID: 33469716
DOI: 10.1007/s00438-020-01756-9

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