Article
Hybridization Capture Using RAD Probes (hyRAD), a New Tool for Performing Genomic Analyses on Collection Specimens.
PloS one - 1 Jan 2016
Suchan Tomasz, Pitteloud Camille, Gerasimova Nadezhda S, Kostikova Anna, Schmid Sarah, Arrigo Nils, Pajkovic Mila, Ronikier Michał, Alvarez Nadir
Abstract excerpt
In the recent years, many protocols aimed at reproducibly sequencing reduced-genome subsets in non-model organisms have been published. Among them, RAD-sequencing is one of the most widely used. It relies on digesting DNA with specific restriction enzymes and performing size selection on the resulting fragments. Despite its acknowledged utility, this method is of limited use with degraded DNA samples, such as...
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