Amplification biases and consistent recovery of loci in a double-digest RAD-seq protocol.

doi:10.1371/journal.pone.0106713

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Article

Amplification biases and consistent recovery of loci in a double-digest RAD-seq protocol.

PloS one - 1 Jan 2014

DaCosta Jeffrey M, Sorenson Michael D

Abstract excerpt

A growing variety of "genotype-by-sequencing" (GBS) methods use restriction enzymes and high throughput DNA sequencing to generate data for a subset of genomic loci, allowing the simultaneous discovery and genotyping of thousands of polymorphisms in a set of multiplexed samples. We evaluated a "double-digest" restriction-site associated DNA sequencing (ddRAD-seq) protocol by 1) comparing results for a zebra finch...

Topics

Animals
Base Composition
Bias
Chromosome Mapping
Computational Biology
Finches
Genetic Loci
Genome
High-Throughput Nucleotide Sequencing
Humans
Nucleic Acid Amplification Techniques
Passeriformes
Polymorphism, Single Nucleotide

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Identifiers

PMID: 25188270
DOI: 10.1371/journal.pone.0106713
PMCID: PMC4154734

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