Article
Reprogramming of sugar transport pathways in Escherichia coli using a permeabilized SecY protein-translocation channel.
Biotechnology and bioengineering - 1 Jun 2020
Guo Qiang, Mei Sen, Xie Chong, Mi Hao, Jiang Yang, Zhang Shi-Ding, Tan Tian-Wei, Fan Li-Hai
Abstract excerpt
In the initial step of sugar metabolism, sugar-specific transporters play a decisive role in the passage of sugars through plasma membranes into cytoplasm. The SecY complex (SecYEG) in bacteria forms a membrane channel responsible for protein translocation. The present work shows that permeabilized SecY channels can be used as nonspecific sugar transporters in Escherichia coli. SecY with the plug domain deleted...
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