Article
An Engineered Survival-Selection Assay for Extracellular Protein Expression Uncovers Hypersecretory Phenotypes in Escherichia coli.
ACS synthetic biology - 19 May 2017
Natarajan Aravind, Haitjema Charles H, Lee Robert, Boock Jason T, DeLisa Matthew P
Abstract excerpt
The extracellular expression of recombinant proteins using laboratory strains of Escherichia coli is now routinely achieved using naturally secreted substrates, such as YebF or the osmotically inducible protein Y (OsmY), as carrier molecules. However, secretion efficiency through these pathways needs to be improved for most synthetic biology and metabolic engineering applications. To address this challenge, we...
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