Article
Ethyl Methanesulfonate Treatment of Maize Pollen for Development of Segregating Mutant Populations or Targeted Mutagenesis.
Cold Spring Harbor protocols - 1 Apr 2026
Khangura Rajdeep S, Best Norman B, Dilkes Brian P
Abstract excerpt
In maize, abundant pollen production and easy controlled pollination permit the direct mutagenesis of pollen to produce populations of independent mutant lines. Pollen can be treated with alkylating agents, such as ethyl methanesulfonate (EMS), to induce point mutations. The ease of applying and decontaminating this mutagen after the mutagenesis application and the advantages provided by the mutation spectra for...
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