Ethyl Methanesulfonate Treatment of Maize Seed for Recovery of Vegetative Mutant Sectors and Segregating Germinal Mutations.

Abstract excerpt

Seed mutagenesis using alkylating chemical agents such as ethyl methanesulfonate (EMS) can generate somatic and germinal mutations in many plant species. In monoecious plants like maize, the sperm- and egg-producing reproductive germlines are derived from distinct cell lineages in the embryo. This separation results in independent mutations inherited via the egg and sperm lineages and prevents the recovery of...

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