Isolation of a thermostable variant of Lip2 lipase from Yarrowia lipolytica by directed evolution and deeper insight into the denaturation mechanisms involved.

Abstract excerpt

Lip2 lipase from Yarrowia lipolytica is a very promising lipase with many potential applications (e.g. resolution of racemic mixtures, production of fine chemicals). Unfortunately this potential is impeded by a very low thermostability for temperatures higher than 40°C. Error-prone PCR and screen...

Topics

• Enzyme Stability
• Fungal Proteins
• Gene Library
• Lipase
• Mutagenesis
• Mutation
• Protein Denaturation
• Temperature
• Yarrowia