Article
The yeast Yarrowia lipolytica as a generic tool for molecular evolution of enzymes.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2012
Duquesne Sophie, Bordes Florence, Fudalej Franck, Nicaud Jean-Marc, Marty Alain
Abstract excerpt
It has been 20 years since strains of the yeast Yarrowia lipolytica were developed for the expression of recombinant proteins as alternative host to the commonly used yeasts, Pichia pastoris and Saccharomyces cerevisiae. Recently, a new strain, JMY1212, was engineered for protein evolution. With...
Topics
- Directed Molecular Evolution
- Fungal Proteins
- Gene Expression
- Genetic Vectors
- Genome, Fungal
- Lipase
- Mutagenesis, Site-Directed
- Mutation
- Oleic Acid
- Plasmids
- Reproducibility of Results
- Transformation, Genetic