Article
An enhanced process for the production of a highly purified extracellular lipase in the non-conventional yeast Yarrowia lipolytica.
Applied biochemistry and biotechnology - 1 Mar 2010
Turki Saoussen, Ayed Atef, Chalghoumi Néjib, Weekers Frederic, Thonart Philippe, Kallel Héla
Abstract excerpt
Yarrowia lipolytica LgX64.81 is a non-genetically modified mutant that was previously identified as a promising microorganism for extracellular lipase production. In this work, the development of a fed-batch process for the production of this enzyme in this strain was described. A lipolytic activity of 2,145 U/mL was obtained after 32 h of batch culture in a defined medium supplemented with 10 g/L of tryptone, an...
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