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Article

CRISPR-Cas9/phosphoproteomics identifies multiple noncanonical targets of myosin light chain kinase.

American journal of physiology. Renal physiology - 1 Mar 2020

Isobe Kiyoshi, Raghuram Viswanathan, Krishnan Laya, Chou Chung-Lin, Yang Chin-Rang, Knepper Mark A

Abstract excerpt

Prior studies have implicated myosin light chain kinase (MLCK) in the regulation of aquaporin-2 (AQP2) in the renal collecting duct. To discover signaling targets of MLCK, we used CRISPR-Cas9 to delete the MLCK gene (Mylk) to obtain MLCK-null mpkCCD cells and carried out comprehensive phosphoproteomics using stable isotope labeling with amino acids in cell culture for quantification. Immunocytochemistry and...

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Identifiers

PMID: 31904282
DOI: 10.1152/ajprenal.00431.2019
PMCID: PMC7099502

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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