Article
Is myosin light-chain phosphorylation a regulatory signal for the osmotic activation of the Na+-K+-2Cl- cotransporter?
American journal of physiology. Cell physiology - 1 Jul 2005
Di Ciano-Oliveira Caterina, Lodyga Monika, Fan Lingzhi, Szászi Katalin, Hosoya Hiroshi, Rotstein Ori D, Kapus András
Abstract excerpt
Myosin light-chain (MLC) kinase (MLCK)-dependent increase in MLC phosphorylation has been proposed to be a key mediator of the hyperosmotic activation of the Na+-K+-2Cl- cotransporter (NKCC). To address this hypothesis and to assess whether MLC phosphorylation plays a signaling or permissive role in NKCC regulation, we used pharmacological and genetic means to manipulate MLCK, MLC phosphorylation, or myosin...
Topics
- Animals
- Azepines
- Carbazoles
- Chlorides
- Enzyme Inhibitors
- Hypertonic Solutions
- Indole Alkaloids
- Kidney Cortex
- Kidney Tubules
- LLC-PK1 Cells
- Methods
- Mutation
- Myosin Light Chains
- Myosins