Article
Enhancement of Z-aspartame synthesis by rational engineering of metalloprotease.
Food chemistry - 1 Jul 2018
Zhu Fucheng, Jiang Tianyue, Wu Bin, He Bingfang
Abstract excerpt
Metalloprotease PT121Y114S, an effective catalyst for Z-aspartame synthesis under the substrate (Z-Asp:l-Phe-OMe) molar ratio of 1:5, was obtained previously. Herein, a computational strategy combining molecular dynamics simulation of the enzyme-substrate complex with binding free energy (ΔG) calculations was established to guide the further engineering of PT121Y114S. One His224 residue proximal to the PT121Y114S...
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