Article
Characterization, stability improvement, and bread baking applications of a novel cold-adapted glucose oxidase from Cladosporium neopsychrotolerans SL16.
Food chemistry - 25 Apr 2020
Ge Jianzhong, Jiang Xiao, Liu Weina, Wang Yuan, Huang Huoqing, Bai Yingguo, Su Xiaoyun, Yao Bin, Luo Huiying
Abstract excerpt
Glucose oxidases are widely used in various industrial processes, including bread baking. In this study, a novel glucose oxidase gene, CngoxA, from Cladosporium neopsychrotolerans SL16, was cloned and expressed in Pichia pastoris. Recombinant CnGOXA exhibited maximal activity at 20 °C and pH 7.0, and was stable at 30 °C and pH 6.0-9.0 for 1 h, with a half-life of 15 min at 40 °C. Compared with CnGOXA, the...
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