Article
A novel approach to improve poly-γ-glutamic acid production by NADPH Regeneration in Bacillus licheniformis WX-02.
Scientific reports - 23 Feb 2017
Cai Dongbo, He Penghui, Lu Xingcheng, Zhu Chengjun, Zhu Jiang, Zhan Yangyang, Wang Qin, Wen Zhiyou, Chen Shouwen
Abstract excerpt
Poly-γ-glutamic acid (γ-PGA) is an important biochemical product with a variety of applications. This work reports a novel approach to improve γ-PGA through over expression of key enzymes in cofactor NADPH generating process for NADPH pool. Six genes encoding the key enzymes in NADPH generation were over-expressed in the γ-PGA producing strain B. licheniformis WX-02. Among various recombinants, the strain...
Topics
- Acetoin
- Bacillus licheniformis
- Bacterial Proteins
- Butylene Glycols
- Gene Expression Regulation, Bacterial
- Glucose
- Glucosephosphate Dehydrogenase
- Kinetics
- Metabolic Networks and Pathways
- Mutation