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Article

Improving the Catalytic Efficiency of Aspergillus fumigatus Glucoamylase toward Raw Starch by Engineering Its N-Glycosylation Sites and Saturation Mutation.

Journal of agricultural and food chemistry - 5 Oct 2022

Song Weiyan, Li Yangyang, Tong Yi, Li Yi, Tao Jin, Rao Shengqi, Li Jianghua, Zhou Jingwen, Liu Song

Abstract excerpt

Raw starch glucoamylase (RSGA) can degrade the raw starch below the starch gelatinization temperature. In this study, to improve the catalytic activity of raw corn starch, N-glycosylation was introduced into the RSGA from Aspergillus fumigatus through site-directed mutation and the recombinant expression in Komagataella phaffii. Among them, the mutants G101S (N99-L100-S101) and Q113T (N111-S112-T113) increased...

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Identifiers

PMID: 36154122
DOI: 10.1021/acs.jafc.2c04733

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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