Article
Coupling chemical mutagenesis to next generation sequencing for the identification of drug resistance mutations in Leishmania.
Nature communications - 9 Dec 2019
Bhattacharya Arijit, Leprohon Philippe, Bigot Sophia, Padmanabhan Prasad Kottayil, Mukherjee Angana, Roy Gaétan, Gingras Hélène, Mestdagh Anais, Papadopoulou Barbara, Ouellette Marc
Abstract excerpt
Current genome-wide screens allow system-wide study of drug resistance but detecting small nucleotide variants (SNVs) is challenging. Here, we use chemical mutagenesis, drug selection and next generation sequencing to characterize miltefosine and paromomycin resistant clones of the parasite Leishmania. We highlight several genes involved in drug resistance by sequencing the genomes of 41 resistant clones and by...
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