CRISPR-Cas9 high-throughput screening to study drug resistance in Leishmania infantum

Abstract excerpt

ABSTRACT Parasites of the genus Leishmania pose a global health threat with limited treatment options. New drugs are urgently needed, and genomic screens have the potential to accelerate target discovery, mode of action, and resistance mechanisms against these new drugs. We describe here our effort in developing a genome-wide CRISPR-Cas9 screen in Leishmania , an organism lacking a functional nonhomologous end...