Article
Impact of quality trimming on the efficiency of reads joining and diversity analysis of Illumina paired-end reads in the context of QIIME1 and QIIME2 microbiome analysis frameworks.
BMC bioinformatics - 15 Nov 2019
Mohsen Attayeb, Park Jonguk, Chen Yi-An, Kawashima Hitoshi, Mizuguchi Kenji
Abstract excerpt
BACKGROUND: To increase the accuracy of microbiome data analysis, solving the technical limitations of the existing sequencing machines is required. Quality trimming is suggested to reduce the effect of the progressive decrease in sequencing quality with the increased length of the sequenced library. In this study, we examined the effect of the trimming thresholds (0-20 for QIIME1 and 0-30 for QIIME2) on the...
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