Article

Optimal 16S rRNA gene amplicon sequencing analysis for oral microbiota to avoid the potential bias introduced by trimming length, primer, and database

2023-07-10

Abstract excerpt

<h4>Background: </h4> . 16S rRNA gene amplicon sequencing analysis is widely used to investigate the diversity and complexity of bacterial communities in the environment. However, the bacterial composition estimated from the experimental data can differ from the original composition. Such a bias occurs depending on methodological stages, including trimming length, selected amplification regions, and referenced dat...

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Literature Corpus work: f7705fe4-6bac-5609-88b9-c56829a77b61
DOI: 10.21203/rs.3.rs-3139837/v1

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