Article
Technical advance in targeted NGS analysis enables identification of lung cancer risk-associated low frequency TP53, PIK3CA, and BRAF mutations in airway epithelial cells.
BMC cancer - 11 Nov 2019
Craig Daniel J, Morrison Thomas, Khuder Sadik A, Crawford Erin L, Wu Leihong, Xu Joshua, Blomquist Thomas M, Willey James C
Abstract excerpt
BACKGROUND: Standardized Nucleic Acid Quantification for SEQuencing (SNAQ-SEQ) is a novel method that utilizes synthetic DNA internal standards spiked into each sample prior to next generation sequencing (NGS) library preparation. This method was applied to analysis of normal appearing airway epithelial cells (AEC) obtained by bronchoscopy in an effort to define a somatic mutation field effect associated with...
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