Article
An Alternative Hot Start PCR Method Using a Nuclease-Deficient ExoIII from Escherichia coli.
Molecular biotechnology - 1 Dec 2019
Lu Shuhong, Zhang Xuesong, Chen Kaiying, Xie Bingbin, Shan Dapeng, Shen Yulong, Li Zhuo
Abstract excerpt
The Hot Start polymerase chain reaction (Hot Start PCR) is designed to reduce off-target amplification by blocking DNA polymerase extension at room temperature until the desired temperature is reached. In this study, we investigated a new method of Hot Start PCR that uses a modified Escherichia coli Exonuclease III (EcoExoIIIM) by substituting residues in the DNA-binding pocket and catalytic center. The results...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.