Article
Specific chromosomal sites enhancing homologous recombination in Escherichia coli mutants defective in RNase H.
Molecular & general genetics : MGG - 1 Sept 1993
Nishitani H, Hidaka M, Horiuchi T
Abstract excerpt
To clone new replication origin(s) activated under RNase H-defective (rnh-) conditions in Escherichia coli cells, whole chromosomal DNA digested with EcoRI was to with a Kmr DNA fragment and transformed into an rnh- derivative host. From the Kmr transformants, we obtained eight kinds of plasmid-l...
Topics
- Chromosomes, Bacterial
- Cloning, Molecular
- DNA Replication
- DNA, Bacterial
- Escherichia coli
- Kanamycin
- Mutation
- Plasmids
- Recombination, Genetic
- Ribonuclease H