Article
Specific chromosomal sites enhancing homologous recombination in Escherichia coli mutants defective in RNase H.
Molecular & general genetics : MGG - 1 Sept 1993
Nishitani H, Hidaka M, Horiuchi T
Abstract excerpt
To clone new replication origin(s) activated under RNase H-defective (rnh-) conditions in Escherichia coli cells, whole chromosomal DNA digested with EcoRI was to with a Kmr DNA fragment and transformed into an rnh- derivative host. From the Kmr transformants, we obtained eight kinds of plasmid-like DNA, each of which contained a specific DNA fragment, termed "Hot", derived from the E. coli genome. Seven of the...
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