Article
Off-target RNA mutation induced by DNA base editing and its elimination by mutagenesis.
Nature - 1 Jul 2019
Zhou Changyang, Sun Yidi, Yan Rui, Liu Yajing, Zuo Erwei, Gu Chan, Han Linxiao, Wei Yu, Hu Xinde, Zeng Rong, Li Yixue, Zhou Haibo, Guo Fan, Yang Hui
Abstract excerpt
Recently developed DNA base editing methods enable the direct generation of desired point mutations in genomic DNA without generating any double-strand breaks1-3, but the issue of off-target edits has limited the application of these methods. Although several previous studies have evaluated off-target mutations in genomic DNA4-8, it is now clear that the deaminases that are integral to commonly used DNA base...
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