[Allelic exclusion in hybridomas. II. Characteristics of the synthesis of 2 allelic variants of rat immunoglobulin kappa-chains in a cloned hybridoma line].

Abstract excerpt

Production of Igk-1a and Igk-1b allelic variants of rat Ig kappa-chains in hybridoma clone 4C2 was studied. Gel-filtration of the culture fluid demonstrates that the cells secrete isolated L-chains in monomeric and dimeric form. Different L-chain allotypes fail to associate in dimers. Immunofluorescence studies prove that both allelic variants are produced in a single cell. Cells with double allotype production...

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