Article
[Analysis of the frequency of allelic exclusion of immunoglobulin light chain genes and molecular characteristics of immunoglobulins secreted by hybridomas with expression of both allelic genes].
Molekuliarnaia biologiia - 1 Jan 2000
Cherepakhin V V, Iakubov L Z, Ibragimov A R, Vengerova T I, Rokhlin O V
Abstract excerpt
The investigation of 750 B-lymphocyte hybridoma clones obtained by fusion of mouse myeloma and newborn heterozygous Igk-la/Igk-1b rat splenocytes has revealed that 9,8% of Ig kappa-chain genes are rearranged productively. Seventeen hybridomas secrete kappa-chains of both allelic variants. The ana...
Topics
- Alleles
- Animals
- Fluorescent Antibody Technique
- Hybridomas
- Immunoglobulin Allotypes
- Immunoglobulin Heavy Chains
- Immunoglobulin Light Chains
- Peptides
- Radioimmunoassay
- Rats