Re-organization of the immunoglobulin kappa gene on both alleles is not an obligatory prerequisite for Ig lambda gene expression in human cells.

Abstract excerpt

Re-arrangement and expression of the immunoglobulin (Ig) genes in B cells occurs under an ordered developmental control. The sequential model of Ig light chain exclusion predicts that only after non-productive re-organization or deletion of both Ig kappa alleles would re-arrangement of Ig lambda gene segments occur. To prove this model, we asked whether expression of Ig lambda light chains is always associated...

Topics

• Alleles
• Blotting, Northern
• Blotting, Southern
• Cell Line
• Gene Expression
• Gene Rearrangement, B-Lymphocyte, Light Chain
• Humans
• Immunoglobulin lambda-Chains