Article
[Detection and preliminary characteristics of the transforming gene (oncogene) of Ha-ras type in human stomach cancer].
Molekuliarnaia biologiia - 1 Jan 2000
Kniazev P G, Schäfer R, Kalinovskiĭ V P, Matveev B V, Mel'nikov R A
Abstract excerpt
High molecular weight DNA prepared from three undifferentiated human stomach carcinomas was assayed for transforming activity by transfection of mouse NIH 3T3 cells. One tumor DNA sample (stomach carcinoma CaVSt) induced (the transfection efficiency: 0.02 transformants/micrograms DNA X 10(-6) cells) transformation of NIH 3T3 recipient cells. Transforming gene of Ha-ras type was identified in transformants derived...
Topics
- Adenocarcinoma
- Animals
- Cell Line
- Cell Transformation, Neoplastic
- Codon
- DNA Restriction Enzymes
- DNA, Neoplasm
- Humans
- Mice
- Mutation
- Oncogenes