Article
[Transformation of NIH/3T3 cells and SW 480 cells displaying K-ras mutation].
Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie - 1 Oct 1994
Anker P, Lyautey J, Lefort F, Lederrey C, Stroun M
Abstract excerpt
The spontaneous release of a glyconucleoprotein complex in the supernatant of eukaryote cell cultures is a general phenomenon independent of cell lysis. The DNA recovered from this glyconucleoprotein material contains most part of the genome. The SW 480 cell line, originating from a human colon carcinoma, presents a point mutation of the K-ras gene on both alleles. These cells in culture release the mutated K-ras...
Topics
- Animals
- Carcinoma
- Cell Line, Transformed
- Colonic Neoplasms
- Fibroblasts
- Genes, ras
- Humans
- Mice
- Mutation
- Polymerase Chain Reaction
- Transfection
- Tumor Cells, Cultured