Article
Multiple Optimal Phenotypes Overcome Redox and Glycolytic Intermediate Metabolite Imbalances in Escherichia coli pgi Knockout Evolutions.
Applied and environmental microbiology - 1 Oct 2018
McCloskey Douglas, Xu Sibei, Sandberg Troy E, Brunk Elizabeth, Hefner Ying, Szubin Richard, Feist Adam M, Palsson Bernhard O
Abstract excerpt
A mechanistic understanding of how new phenotypes develop to overcome the loss of a gene product provides valuable insight on both the metabolic and regulatory functions of the lost gene. The pgi gene, whose product catalyzes the second step in glycolysis, was deleted in a growth-optimized Escherichia coli K-12 MG1655 strain. The initial knockout (KO) strain exhibited an 80% drop in growth rate that was largely...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.