Article
"NAD-capQ" detection and quantitation of NAD caps.
RNA (New York, N.Y.) - 1 Oct 2018
Grudzien-Nogalska Ewa, Bird Jeremy G, Nickels Bryce E, Kiledjian Megerditch
Abstract excerpt
RNA 5' cap structures comprising the metabolic effector nicotinamide adenine dinucleotide (NAD) have been identified in diverse organisms. Here we report a simple, two-step procedure to detect and quantitate NAD-capped RNA, termed "NAD-capQ." By use of NAD-capQ we quantitate NAD-capped RNA levels in Escherichia coli, Saccharomyces cerevisiae, and human cells, and we measure increases in NAD-capped RNA levels in...
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