Article
T-DNA Insertional Mutagenesis and Activation Tagging in Medicago truncatula.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018
Panara Francesco, Calderini Ornella, Porceddu Andrea
Abstract excerpt
The development of plant genetic transformation techniques has greatly enhanced our capacity to investigate and understand gene function. Since T-DNA constructs insert randomly in genomes, in principle, it is possible to construct a population of individuals harboring one or more T-DNA inserted in any region of the genome. Such populations can be screened following two approaches: (1) given a mutant phenotype,...
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