Highly Efficient Gene Knockout in Medicago truncatula Genotype R108 Using CRISPR-Cas9 System and an Optimized Agrobacterium Transformation Method.

doi:10.1007/978-1-0716-3131-7_15

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Article

Highly Efficient Gene Knockout in Medicago truncatula Genotype R108 Using CRISPR-Cas9 System and an Optimized Agrobacterium Transformation Method.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2023

Lawrenson Tom, Atkinson Nicola, Forner Macarena, Harwood Wendy

Abstract excerpt

Medicago truncatula is the model plant species for studying symbioses with nitrogen-fixing rhizobia and arbuscular mycorrhizae, where edited mutants are invaluable for elucidating the contributions of known genes in these processes. Streptococcus pyogenes Cas9 (SpCas9)-based genome editing is a facile means of achieving loss of function, including where multiple gene knockouts are desired in a single generation....

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PMID: 36995630
DOI: 10.1007/978-1-0716-3131-7_15

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