Article
Targeting Induced Local Lesions IN Genomes (TILLING) in Medicago truncatula.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018
Sánchez Myriam, Le Signor Christine, Aubert Gregoire, Darchy Brigitte, Gallardo Karine, Thompson Richard D
Abstract excerpt
TILLING is a reverse genetics strategy that combines the high density of point mutations provided by traditional chemical mutagenesis with rapid screening of DNA pools from a mutagenized population for induced mutations (McCallum et al., Nat Biotechnol 18:455-457, 2000). This high-throughput technique allows the identification of point mutations in any gene of interest.
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