Article
Reverse genetics in Medicago truncatula using a TILLING mutant collection.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2013
Carelli Maria, Calderini Ornella, Panara Francesco, Porceddu Andrea, Losini Ilaria, Piffanelli Pietro, Arcioni Sergio, Scotti Carla
Abstract excerpt
Medicago truncatula is one of the model species for legume molecular genetics. In the last decade different types of mutant populations have been created in this species that can be screened by forward and reverse-genetic approaches to identify and functionally characterize genes of interest. TILLING is a reverse-genetic method combining random chemical mutagenesis and a PCR-based screen to identify point...
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