Article
High-throughput generation of an activation-tagged mutant library for functional genomic analyses in tobacco.
Planta - 1 Mar 2015
Liu Feng, Gong Daping, Zhang Qian, Wang Dawei, Cui Mengmeng, Zhang Zhiguo, Liu Guanshan, Wu Jinxia, Wang Yuanying
Abstract excerpt
Tobacco (Nicotiana tabacum L.) is an ideal model system for molecular biological and genetic studies. In this study, activation tagging was used to generate approximately 100,000 transgenic tobacco plants. Southern blot analysis indicated that there were 1.6 T-DNA inserts per line on average in our transformed population. The phenotypes observed include abnormalities in leaf and flower morphology, plant height,...
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