Article
Large-scale insertional mutagenesis using the Tnt1 retrotransposon in the model legume Medicago truncatula.
The Plant journal : for cell and molecular biology - 1 Apr 2008
Tadege Million, Wen Jiangqi, He Ji, Tu Haidi, Kwak Younsig, Eschstruth Alexis, Cayrel Anne, Endre Gabriella, Zhao Patrick X, Chabaud Mireille, Ratet Pascal, Mysore Kirankumar S
Abstract excerpt
Medicago truncatula is a fast-emerging model for the study of legume functional biology. We used the tobacco retrotransposon Tnt1 to tag the Medicago genome and generated over 7600 independent lines representing an estimated 190,000 insertion events. Tnt1 inserted on average at 25 different locations per genome during tissue culture, and insertions were stable during subsequent generations in soil. Analysis of...
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