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Article

Testing the '+2 rule' for lipoprotein sorting in the Escherichia coli cell envelope with a new genetic selection.

Molecular microbiology - 1 Nov 1999

Seydel A, Gounon P, Pugsley A P

Abstract excerpt

We report a novel strategy for selecting mutations that mislocalize lipoproteins within the Escherichia coli cell envelope and describe the mutants obtained. A strain carrying a deletion of the chromosomal malE gene, coding for the periplasmic maltose-binding protein (MalE), cannot use maltose unless a wild-type copy of malE is present in trans. Replacement of the natural signal peptide of preMalE by the signal...

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Source

Identifiers

PMID: 10564520
DOI: 10.1046/j.1365-2958.1999.01647.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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