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Article

Destabilizing interactions between the partners of a bifunctional fusion protein.

Protein engineering - 1 Feb 1996

Blondel A, Nageotte R, Bedouelle H

Abstract excerpt

Hybrid MalE-GVP is a bifunctional protein in vitro since it binds maltose as protein MalE of Escherichia coli and since it is dimeric and specifically binds single-stranded DNA as protein GVP of phage M13. The oxidation rate of a unique cysteine residue was used to compare the stabilities of GVP in its free and hybrid forms, under conditions where MalE was either folded or unfolded by a denaturing agent. The...

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Source

Identifiers

PMID: 9005445
DOI: 10.1093/protein/9.2.231

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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