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Multiplex PCR and Nanopore Sequencing of Genes Associated with Antimicrobial Resistance in Neisseria gonorrhoeae Directly from Clinical Samples

20 Nov 2020

Abstract excerpt

BACKGROUND: Antimicrobial resistance (AMR) of Neisseria gonorrhoeae has spread worldwide. Rapid and comprehensive methods are needed to describe N. gonorrhoeae AMR profiles accurately. A method based on multiplex amplicon sequencing was developed to simultaneously sequence 13 genes related to AMR in N. gonorrhoeae directly from clinical samples. METHODS: Nine N. gonorrhoeae strains were used for the establishment...

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Bacterial Infections and Vaccines
Neonatal and Maternal Infections
Reproductive tract infections research

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 33367585
DOI: 10.1093/clinchem/hvaa306

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