Article
Context-dependent expression of a conditionally-inducible form of active Akt.
PloS one - 1 Jan 2018
Park Soyeon, Burke Robert E, Kareva Tatyana, Kholodilov Nikolai, Aimé Pascaline, Franke Thomas F, Levy Oren, Greene Lloyd A
Abstract excerpt
Akt kinases are key signaling components in proliferation-competent and post-mitotic cells. Here, we sought to create a conditionally-inducible form of active Akt for both in vitro and in vivo applications. We fused a ligand-responsive Destabilizing Domain (DD) derived from E. coli dihydrofolate reductase to a constitutively active mutant form of Akt1, Akt(E40K). Prior work indicated that such fusion proteins may...
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