Article
A hybrid recognition sequence in a recombinant restriction enzyme and the evolution of DNA sequence specificity.
Nature - 1 Jan 2000
Nagaraja V, Shepherd J C, Bickle T A
Abstract excerpt
Early attempts to generate new restriction specificities by recombination between allelic restriction-modification systems have been unsuccessful. Bullas et al. succeeded in isolating a new specificity, SQ, in Salmonella that they interpreted as being the result of a recombination event between t...
Topics
- Alleles
- Bacterial Proteins
- Base Sequence
- Binding Sites
- DNA (Cytosine-5-)-Methyltransferases
- DNA Restriction Enzymes
- DNA, Bacterial
- DNA, Fungal
- DNA, Viral
- Deoxyribonucleases, Type I Site-Specific
- Plasmids
- Recombination, Genetic
- Salmonella
- Salmonella typhimurium
- Substrate Specificity